Nearly every aspect of neuronal function depends on the accurate transport of membrane proteins to axons or dendrites. Axons conduct electrochemical action potentials and primarily send signals, whereas dendrites primarily receive signals. Because these two domains have distinctly different functions, they each require a specific complement of membrane proteins. These proteins are delivered to their cargo by vesicle transport. Vesicle carriers that contain axonal proteins are transported to the axon; vesicle carriers that contain dendritic proteins are transported to dendrites and excluded from the axon. This phenomenon of directed vesicle movement to dendrites or the axon is referred to as selective transport.
Understanding the molecular regulation of vesicle transport is a fundamental problem in neuroscience and cell biology and defects in vesicle transport are thought to underlie the axonal degeneration characteristic of many neurodegenerative diseases. Intracellular carriers are moved by motor proteins that generate locomotive force. One family of motor proteins are kinesins. Our long-term goal is to determine the molecular regulation of kinesin motors and understand how they confer specific transport behaviors to vesicles that move cargoes to axons and dendrites.
Our lab uses a variety of cell biological approaches to understand the molecular mechanisms that underlie intracellular vesicle transport. Primarily, we utilize live-cell fluorescent microscopy to track and measure individual vesicle carriers as they move inside cells. Our experiments also rely heavily on molecular biology, biochemistry, and tissue culture techniques.
Dr. Bentley received his Ph.D. in 2010 in the laboratory of Jesse Hay at the University of Montana and completed a postdoc with Gary Banker at Oregon Health & Science University. He joined the faculty in the Department of Biological Sciences at the Rensselaer Polytechnic Institute in the summer of 2017.
Ph.D., University of Montana, Cell Biology.
Postdoctoral training, Oregon Healthy & Science University, Neuroscience and Cell Biology.
- Yang, R., Z. Bostick, A. Garbouchian, J. Luisi, G. Banker, and M. Bentley. 2019. A novel strategy to visualize vesicle-bound kinesins reveals the diversity of kinesin-mediated transport. Traffic. 20: 851– 866. https://doi-org.libproxy.rpi.edu/10.1111/tra.12692.
- Bentley, M., and G. Banker. 2016. The cellular mechanisms that maintain neuronal polarity. Nat Rev Neurosci. 17:611–622. doi:10.1038/nrn.2016.100.
- Bentley, M., H. Decker, J. Luisi, and G. Banker. 2015. A novel assay reveals preferential binding between Rabs, kinesins, and specific endosomal subpopulations. J Cell Biol. 93:4604. doi:10.1083/jcb.201408056.
- Bentley, M., and G. Banker. 2015. A Novel Assay to Identify the Trafficking Proteins that Bind to Specific Vesicle Populations. Curr Protoc Cell Biol. 69:13.8.1–13.8.12. doi:10.1002/0471143030.cb1308s69.
- Yang, R., M. Bentley, C.F. Huang, and G. Banker. 2015. Analyzing kinesin motor domain translocation in cultured hippocampal neurons. Methods Cell Biol. doi:10.1016/bs.mcb.2015.06.021.
- Jenkins, B., H. Decker, M. Bentley, J. Luisi, and G. Banker. 2012. A novel split kinesin assay identifies motor proteins that interact with distinct vesicle populations. J Cell Biol. 198:749–761. doi:10.1083/jcb.201205070.
- Helm, J.R., M. Bentley, K.D. Thorsen, T. Wang, L. Foltz, V. Oorschot, J. Klumperman, and J.C. Hay. 2014. Apoptosis Linked Gene-2 (ALG-2)/Sec31 Interactions Regulate ER-to-Golgi Transport: a Potential Effector Pathway for Luminal Calcium. J. Biol. Chem. jbc.M114.561829. doi:10.1074/jbc.M114.561829.
- Bentley, M., J. Helm, J. Klumperman, and J.C. Hay. 2011. Luminal calcium regulates ER/Golgi anterograde transport efficiency through ALG-2/SEC31 interactions.
- Bentley, M., D.C. Nycz, A. Joglekar, I. Fertschai, R. Malli, W.F. Graier, and J.C. Hay. 2010. Vesicular calcium regulates coat retention, fusogenicity, and size of pre-Golgi intermediates. Mol Biol Cell. 21:1033–1046. doi:10.1091/mbc.E09-10-0914.
- Bentley, M., Y. Liang, K. Mullen, D. Xu, E. Sztul, and J.C. Hay. 2006. SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion. J. Biol. Chem. 281:38825–38833. doi:10.1074/jbc.M606044200.